![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4092110:Design
Designed by: Shani Betsymar Leyva Camacho & Damian Alonso Espinoza Ruiz Group: iGEM21_Tec-Monterrey (2021-10-20)
Toehold Fusarium 1 expression cassette with mRFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 681
Illegal AgeI site found at 793 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This cassette expression is regulated by a T7 promoter and expresses a red fluorescent protein when the toehold interacts with its corresponding trigger sequence.
Source
Toehold RNA complementary to 28S-18S ribosomal RNA intergenic spacer from Fusarium oxysporum, T7 promoter from bacteriophage T7 and mRFP from Discosoma striata (coral).